By Carter Litchfield
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Additional info for Analysis of Triglycerides
S e m i l o g plot of retention time vs. chain length for c o d liver phospholipid fatty acid m e t h y l esters o n a capillary c o l u m n coated with butanediol succinate polyester. Parallel linear relationships exist for e a c h h o m o l o g o u s series. T h u s if the retention time of o n e m e m b e r of an h o m o l o g o u s series is k n o w n , the retention time o f a n y other m e m b e r can be accurately predicted. D a t a taken f r o m c h r o m a t o gram in Fig. 2-6. F r o m A c k m a n ( 5 ) .
The technique has been widely used to separate plasma lipids into neutral lipid and phospholipid fractions prior to further analysis. The advantage of single-solvent fractionation is its speed; triglycerides are separated from the more polar lipids in just a few minutes, rather than the 1-3 hours required by column chromatography. The disadvantage of single-solvent fractionation is its inability to separate triglycerides from less polar lipids (hydrocarbons, sterol esters, wax esters, and diacyl glyceryl ethers) which remain with the triglycerides and can interfere with further analyses.
Figure 2-4 shows typical gas chromatograms for isothermal analyses of cod liver oil fatty acid methyl esters on two different polyester liquid phases. C. Identification of Peaks The multiplicity of peaks in most methyl ester gas chromatograms necessitates a systematic approach to peak identification. Seven major techniques 34 2. EXTRACTION, 16 ISOLATION, 18 CHAIN AND MEASUREMENT 20 LENGTH F i g . 2-5. S e m i l o g plot of retention time vs. chain length for c o d liver phospholipid fatty acid m e t h y l esters o n a capillary c o l u m n coated with butanediol succinate polyester.
Analysis of Triglycerides by Carter Litchfield